Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis

The metabolism is reprogrammed in cancer cells to meet the requirement of their malignant properties. Some specific metabolites can contribute transformation different types. This study aimed determine level glucose, pyruvate, lactate, citrate, alpha-ketoglutarate (α-KG), 2-hydroxyglutarate (2-HG), succinate, fumarate, malate, and oxaloacetate tissues (tumor, colon, brain, liver) xenograft models generated by a colon adenocarcinoma cell line (SW480). Glucose pyruvate levels were determined 3,5-dinitrosalicylic acid (DNS) method assay, respectively. other was HPLC analysis. results show that D-2-HG highly produced tumor (120.2 mg/L), but it could not be detected liver tissue. In conclusion, TCA Cori cycles Glycolysis investigated found some tissue-specific.